E6-AP Double Nickase Plasmid [Mouse]
Partner: Santa Cruz Biotechnology
| Species Reactivity: | Mouse |
| Description: | Double Nickase Plasmids consist of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart. Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB. One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection. |
| Further Information: | 20ug of transfection-ready purified plasmid DNA, suitable for up to 20 transfections |
| Shipping Conditions: | Ship at ambient |
| Storage: | Store at -20 C |
| Usage: | Research use only |
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