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Anti-ABCB4 Antibody at 1:4000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 142 kDaBlocking/Dilution buffer: 5% NFDM/TBST.