Gene Silencing Product Ranges

Insight Biotechnology offers a broad range of Gene Silencing products against the entire human mouse and rat genomes, manufactured by Santa Cruz Biotechnology, Origene, Bioo Scientific and Allele Biotechnology.  A brief overview of these ranges is listed below.
Insight Biotechnology’s team of expert scientists is happy to answer any technical or pricing questions relating to these products, so please get in touch, Tel: +44(0)208 385 0303 or email Info@insightbio.com.

Santa Cruz: Post Transcriptional Gene Silencers

Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%) known human and mouse genes. siRNAs are provided as pools of 3 target specific 19-25 nt siRNAs designed to knock down expression of specific gene of interest. Each vial contains 3 nmol of lyophilized siRNA and is sufficient for 50 - 100 transfections. For independent verification of gene silencing results, we also will provide, upon request, individual siRNA duplex components, each containing 3 nmol of lyophilized siRNA. shRNA Plasmid DNA Gene Silencers are provided as pools of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNA (sequences correspond to our well-established existing product line of siRNA gene silencers). shRNA Plasmids are provided as 20 µg transfection-ready plasmid DNA for either transient or stable (puromycin-selection) target specific gene silencing. shRNA Lentiviral Particles are provided as pools of three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin loop) shRNA and are provided as 200 µl transduction-ready viral particles for gene silencing in mammalian cells (human or mouse). Suitable control antibodies and RT-PCR primers are available for monitoring gene expression knockdown.

Origene: HuSH-29: Genome-wide shRNA for Human, Mouse and Rat Genes

HuSH-29 are pre-designed shRNA with genome wide coverage (human, mouse and rat) that delivers guaranteed knockdown. The same superior design is also offered for other species (please enquire). Features and Benefits Three retroviral vector options: pRS: basic retroviral vector without fluorescence pRS with GFP to monitor transfection pRS with RFP, ideal for double knockdown Guaranteed successful gene knockdown (≥70%) Versatile applications: transient or stable transfection or retroviral infection (4 gene-specific shRNA and two negative controls) provided at low prices 5 μg transfection-ready DNA using PowerPrep® HP Kits Trilencer-27 siRNA kits targeting human genes are now available

Double Gene Knockdown Experiment Scrambled shRNA (GFP Vector) shRNA against RFP (GFP Vector) + + Scrambled shRNA (RFP Vector)   shRNA against GFP (RFP Vector)

Allele: RNA Interference
Allele Biotech was one of the very first companies to offer synthetic siRNA and RNA polymerase III promoter systems for expressing shRNA. Allele Biotech has been granted US patents on DNA-based siRNA and shRNA platforms that are popularly used as gene silencing tools. Allele Biotech´s SilenCircle platform design allows high level expression of siRNA or shRNA. RNAi transcripts expressed by this method have sequence identities precisely as desired, i.e. without extra bases introduced by cloning sites, thereby reducing non-specific effects. The LineSilence platform depends on linear DNA cassettes for expressing siRNA, eliminating cloning steps. The AlleleHPLC line of RNAi products offers the best value in synthetic siRNA currently on the market, with guaranteed amount of annealed siRNA, every single siRNA HPLC purified, at prices ~50% of competitors´! Choosing transfection methods appropriate for each type of RNAi reagent is critical for successful gene silencing. The AvantGene Transfection reagent works well for delivering PCR or plasmid DNA reagents, whereas AvantGeneSi is ideal for synthetic siRNA. When primary cells need to be used, the choices for RNAi reagent delivery are normally narrowed down to electroporation or viral vectors, with the latter more widely used. Allele´s RNAi product family includes U6 promoter driven shRNA cassettes (US patents issued and pending) carried on HIV-based lentiviral vector pLICO (implico, Latin for intertwine), and MMLV-based retroviral vector pCHAC (Chac, Mayan for God of culture and growth), with the options of including an IRES-driven mWasabi. Everything you need for RNAi, you can find it here.